MICROBIOLOGY
iBright 1500 Imager
BMI 1200D
Camera
Detector Cooled 16-bit CCD, 65,535 shades of gray
Resolution 9.1 megapixels
Lens Fixed, 25 mm, f/0.95
Field of view 22.5 x 18.0 cm (W x D) (image up to 4 mini blots or gels)
Binning modes 1×1, 2×2, 3×3, 4×4, 5×5, 6×6, 8×8
Zoom 1–8X (1–2X mechanical + 1–4X digital)
Main supported imaging applications:
• Colorimetric stained protein gels
• Fluorescent stained protein gels
• Fluorescent stained nucleic acid gels
• Colorimetric stained membranes
• Chemiluminescent western blots
• Colorimetric western blots
• Fluorescent western blots
Qualitative visible imaging applications:
• Translucent objects (e.g., colony plates)
• Opaque objects (e.g., 2D strips, TLC plates, leaf sections)
• GFP expression in multiwell (e.g., 6-well) plates
Automated features
• Automatic zoom
• Automatic focus
• Automatic exposure (Smart Exposure)
• Automatic on-board image analysis
Filters:
Filter sets: 12 filters (6 excitation, 6 emission)
Illumination sources:
• Green LED (470–550 nm) transilluminator
• Epi white LED
• Epi near-IR LED
QuantStudio 6 Pro RT-PCR
BMI 1200D
Reaction volume: 384-well block: 5–20 μL
Excitation source: Bright white LED
Filter or color combinations: 5
Excitation/emission range: 450–680 nm
Multiplexing: 5 or 6 targets
Maximum block ramp rate: 384-well block: 6.0°C/sec
Average sample ramp rate: 3.66°C/sec
Temperature uniformity: 0.4°C
Temperature range: 4–99.9°C
Compatible dyes: FAM™, SYBR™ Green, VIC™, JOE™, TET™, ABY™, NED™, TAMRA™, Cy®3, JUN™, ROX™, Texas Red™, Mustang Purple™, Cy®5, LIZ™, Cy®5.5
MiniAmp™ Plus Thermocycler
BMI 1200D
Block format: 96-well, 0.2 mL 3-zone VeriFlex Block
Maximum block ramp rate: 3.5°C/sec 3.0°C/sec
Maximum sample ramp rate: 2.7°C/sec 2.2°C/sec
Temperature accuracy: ±0.25°C (35–99.9°C)
Temperature range: 0–100.0°C
Temperature uniformity: <0.5°C (30 sec after reaching 95°C)
Temperature calibration: Calibrated to standards traceable to the National Institute of Standards and Technology
PCR volume range: 10–100 μL
SpectraMax iD3
BMI 1200D
Read modes:
• Absorbance
• Fluorescence (top/bottom read)
• Luminescence (top read)
Wavelength ranges:
• Abs: 230 – 1000 nm
• FL Ex: 250 – 830 nm
• FL Em: 270 – 850 nm
• Lumi: 300 – 850 nm
Wavelength selection:
• Monochromator tunable in 1-nm increments
Absorbance photometric accuracy/linearity:
• < ±0.010 OD ±1.0%, 0 – 3 OD VIS 0 – 3 OD UV
Absorbance photometric precision/repeatability:
• < ±0.003 OD ±1.0%, 0 – 3 OD VIS 0 – 3 OD UV
Sensitivity top read optimized:
• FL: 1 pM fluoresein 96 well, 1 pM fluorescein 384 well
• Lumi: 2 pM ATP 96 well, 4 pM
Light source(s):
• High power xenon flash lamp
Detector(s):
• Silicon photodiode
• Ultra-cooled photomultiplier tube
Read types (endpoint, kinetic, etc):
• Endpoint
• Kinetic
• Spectrum scan
• Well scan
Read times (kinetic interval for 96-well plate)*:
• Abs: 28 seconds
• FL: 21 seconds
• Lumi: 21 seconds
Plate shaking:
• Linear
• Orbital
• Double orbital
Plate type(s):
• 6-384 well plates
Temperature control:
• Ambient + 5°C to 66°C
Validation tools:
• Absorbance validation plate
• Fluorescence validation plate
• Luminescence validation plate
• SoftMax® Pro GxP Software
Thermo Scientific™ NanoDrop™ One/OneC
BMI 1200D
Detection Limits
| Measurement | Location Pathlength (mm) | Upper Detection Limit (10 mm Equivalent Absorbance) |
|---|---|---|
| Pedestal | 1 | 12.5 |
| 0.2 | 62.5 | |
| 0.1 | 150 | |
| 0.05 | 300 | |
| 0.03 | 550 | |
| Cuvette | 10 | 1.5 |
| 5 | 3 | |
| 2 | 7.5 | |
| 1 | 15 |
